Mentik susu rice is a local rice variety from Magelang, Central Java has an advantage but it is not interesting to farmers because of it’s high stem, long life and low yield. One method of using physical mutagen isi gamma ray radiation. The purpose of this research are observing the performance of mutated M5 generation of mentik susu rice which already irradiated with 200 Gy gamma ray and selecting the short-stemmed ones which have high productivity. The research was conducted on Palur Village, sub-district of Mojolaban, Sukoharjo Regency. The experiment was done by planting 35 individual M5 and control seeds, by using simple design experiment. The variables observed were plant height, total number of tillers, number of productive tillers, age of flowering and harvesting, panicle length, number of seeds per panicle, panicle intensity index, weight of 100 seeds, seed yield per plant. Data from the observations were analyzed descriptively and compared the performance of mentik susu rice M5 with control via T test analysis. The results showed that there was a difference in the performance of the M5 mutants of mentik susu rice as a result of 200 Gy gamma ray irradiation compared to control mentik susu rice. There are 22 selected mutant plant individuals in the M-MS2-G15T3-5-2 ( 18,25,16,8) lines; M-MS2-G15T3-4-15 (10) lines; M-MS2-G15T3-2-5 (56, 18, 9) lines; M-MS2-G18T7-4-4 (11) lines; M-MS2-G18T7-4-(15, 13, 18) lines; M-MS2-G17T17-13-9 ( 23, 21, 29) lines; M-MS2-G18T7-4-9 (17 dan 12) lines; M-MS2-G15T3-2-18 (35) lines; M-MS2-G15T3-2-14 (28, 61, 36) lines; and M-MS2-G18T3-6-13 (35) lines.