Growing Artemisia annua in tropical areas cause a short vegetative period and resulted in low content of artemisinin. To increase the artemisinin content of Artemisia annua in the tropical area could be carried out by administering of Nitrogen fertilizer. In order to increase production and extension of grow areas, the selection of superior accession which adaptable in low areas is also needed. The study was carried out using an experimental method by applying 3 levels dosage of Nitrogen fertilization (100, 200 and 300 kg/Ha) to 3 Artemisia annua accessions (V1, V2 and V5). The research was conducted at April-August 2018 at Karangpandan research station with an elevation of 600 m asl. The parameters observed were plant height, stem diameter, number of branches, fresh biomass weight, dry biomass weight, artemisinin content and essential oil content. The results showed that the growth and productivity of 3 accession of Artemisia annua is strongly influenced by the Nitrogen dosage. The lower dosage of Nitrogen decline growth and productivity, and plant entry into the generative phase later for the highest dosage of Nitrogen. The Nitrtogen application didn’t effect on the artemisinin content of Artemisia annua.