Based on data from the Directorate General of Food Crops of the Ministry of Agriculture in 2020 predicted corn of Indonesia has a surplus where the average corn demand is 1.5 million tons/month while the average corn production is 1.65 million tons/month. To face global competition, Indonesia must be able to increase the competitiveness of agricultural products by reducing production costs, increasing quality and productivity and developing environmentally friendly technologies such as the application of liquid organic fertilizer combined with biological fertilizers (BIO-LOF) which can be functioned as organic fertilizers and biological fertilizers. Liquid Organic Fertilizer is produced using cow urine ingredients, Gliricedia and titonia leaves and coconut coir. While the biological fertilizer added is a consortium of bacteria Serratia marcecens, Pseudomnas fluorescens and Bacillus thuringiensis. The research aims to find out the BIO-LOF application technique to increase corn growth and production. The design used is factorial randomized block design (RBD) with 3 replications, where the P Factor (Bio-LOF concentration) with 4 levels, namely P1 = without Bio-LOF, P2 = 25% concentration, P3 = concentration of 50%, and P4 = concentration of 75% ,. Factor W (application time) with 3 levels, namely W1 = treatment of seeds + when planting + age 3 weeks + age 6 weeks, W2 = treatment of seeds + every week, W3 = treatment of seeds + every 2 weeks, so that it is obtained 36 trial plots. The best BIO-LOF application is 50% concentration can increase the number of leaves and increase corn production by 42.2% compared without BIO-LOF application. The best application time is that the seed treatment is repeated at planting time and weeding 1 and weeding 2.