Black rice is one of local rice popular as functional food and potential to be developed because it has benefits for health. The high crop posture, long time period for cultivating, and low productivity being limited factors for cultivating black rice. Induced mutation using irradiation of gamma rays was expected to produce M3 mutant black rice that has better agronomic and physiological properties. The aims of this research were the examine morphophisiological characters by growth analysis and yield performance on M3 generation of local black rice varieties from Boyolali and Bantul as a result of 100 Gy gamma ray irradiation. The research was conducted on a paddy field that located in Jati Village, Karanganyar on April- August 2019. The parameters observed were Leaf Area Index (LAI), Net Assimilation Rate (NAR), Relative Growth Rate (RGR), Specific Leaf Weight (SLW) on 2-4 WAP, plant height, the total number of tillers, the number of productive tillers, panicle density index, number of filled grains per panicle, and weight of grain per clump. The result showed morphophisiological character on M3 mutant black rice of Bantul varieties with dose 100 Gy based on growth analysis showed that the value of NAR, SLW, and RGR is high on 2-4 WAP. The local varieties of black rice on Bantul 0 Gy had a high increase of LAI on 2-4 WAP. Mutation that occurs on mutant M3 black rice at a dose of 100 Gy cause it had a short plant height, but the yield components were still low.