Paper ID: 105
DETECTION OF GROWTH HORMON GENE POLYMORPHISM AND ITS ASSOCIATION WITH BODY WEIGHT OF KEBUMEN-PO CATTLE
Soewandi BDP 1, Hartati 2 and Hapsari AAR1
¹Indonesia Research Institute of Animal Production (IRIAP), Jl. Veteran III, Ciawi 16002, West Java, Indone
² Beef Cattle Research Station, Jl. Pahlawan no. 2 Grati, Pasuruan, East Java 16784, Indonesia
Kebumen-PO cattle is one of local genetic resources that has good body weight and growth. The aim of this research was to detect growth gene polymorphisms (GHR, GH and Pit1) and their associations with body weight of Kebumen-PO cattle. The sample used was 100 heads of Kebumen-PO cattle belonging to Asosiasi Peternakan Sapi PO Kebumen (ASPOKEB) livestock group. This research observed Kebumen-PO cattle growth from birth to one year of age. DNA extraction of Kebumen-PO cattle used blood samples. After DNA extraction, PCR-RFLP method was used to observe three growth genes (GH, GHR and Pit1). This result of this research showed that GH and GHR gene was polymorphic, while the Pit1 gene is monomorphic. The Kebumen-PO cattle has average of birth weight, weaning weight and yearly weight 29.0+0.29 kg, 150.5+5.09 kg and 203+5.62 kg, respectively. The analysis showed that three growth genes were not significantly associated with the body weight of Kebumen-PO cattle.