Pigeonpea has great potential to be alternate source of plant protein beside mungbean, soybean, and ground nut. The potential of pigeonpea needs to be improved by plant breeding program. Genetic informations are needed to improved plant breeding. This research aims to gain genetic information based on chromosome numbers, chromosome shape and chromosome length to visualize pigeonpea karyotypic. The research was conducted at Plant Physiology and Biotechnology Laboratory Faculty of Agriculture UNS and Plant Breeding Laboratory Mendel Room Faculty of Agriculture UGM. This research had be done by making glass slides using squashing method include fixation use Carnoy 1 solution (3 absolute ethanol : 1 96% acetic acid), hydrolysis (1N HCl) and chromosome staining (aceto orcein 2%). Observation used light microscope then measured and analyzed use Photoshop and Image Raster. The results showed that chromosome number of pigeonpea is 2n = 2x = 22 with chromosome length 5.73 ± 1,15 μm  until 10.92 ± 2.69 μm. Shape of pigeonpea chromosomes are metacentric and submetacentric which are metacentric is dominant. Karyotypic formula of pigeon pea is 2n = 2x = 9 m + 2 sm. Intrachromosomal asymmetry index (As1%) of pigeon pea is 59% and Interchromosomal asymmetry index (A2) value of pigeon pea is 0.34 ± 0.02.