Cavendish banana is one type of banana that has a high economic value, especially for food export commodities. High export opportunities need to be balanced with increased productivity by selecting superior seedlings through in vitro seed propagation. The tissue fruitfulness depends on the media used. The study was conducted at the Biotechnology Laboratory of Agriculture Faculty UNS in May to December 2019. Cavendish banana explant which obtained from KBH in Salaman, Magelang. The media used substitution between MS media and Gandasil D leaf fertilizer with different concentrations. The researcher also used a completely randomized design (CRD) of one factor with five treatment combinations and six repetition. The treatment used is G1 = MS 100% as a control; G2 = 75% MS + 25% Gandasil D; G3 = G3 = 50% MS + 50% Gandasil D; G4 = 25% MS + 75% Gandasil D; and G5 = 100% D. the variables observed include time of buds, number of shoots, plantlet height, time of leaves, number of leaves, time of roots, number of roots, and root length. The data analysis used is the physical analysis method based on the F test of 5% level. The results of the research showed that 100% Gandasil D and 25% MS + 75% Gandasil D treatment had the highest influence on the number of leaves, number of shoots, plant height, and number of roots. The mixture of MS media and leaf fertilizer can be used as an alternative medium for multiplication of Cavendish bananas.