Research aims to study the effect of organic fertilizer dosages and  Biofilmed Biofertilizer formula on nitrogen uptake and shallot yield in Alfisols. Field experiment was conducted at Sukosari Village, Jumantono, Karanganyar district, Central Java, Indonesia. The experimental design used was Randomized Completely Block Design with two factors namely dose of organic fertilizers (D₀, D₁, D₂) and Biofilmed biofertilizer formula (F₀, F₁, F₂, F₃).  Each combination treatment repeated three times. Variables observed included N-uptake, plant fresh and dry weight, number and diameter of bulb, and shallot yield. Data was analyzed by F test at 95% of level confidence, followed by Duncan Multiple Range Test (DMRT) if any significant influence.The result showed that organic fertilizer doses influence significantly on bulb weight, while the interaction of organic fertilizer dose and biofertilizer formula influence N uptake. Highest bulb weight achieved by the use of 20 tons ha^-1 organic fertilizer (62,9 g clump^-1), increase 348,65% compared to control treatment (14.04 g clump^-1). The highest N-uptake was taken from the treatment combination of 10 tons ha^-1  organic fertilizer and biofertilizer formula F₁ (0,17 gr N/clumps), increase 183,33% than control treatment (0.06 g clump^-1).