Efforts to propagate banana seeds with good quality on a large scale can be pursued through tissue culture techniques. In tissue culture often uses Murashige and Skoog (MS) media but these media are difficult to obtain. Growmore is a leaf fertilizer that has the potential to replace MS media because it has a complete nutrient content. This study aims to obtain the most effective composition of MS media and growmore fertilizer for the growth of ambon banana shoots in vitro. The study was conducted at the Plant Physiology and Biotechnology Laboratory, Sebelas Maret University using a completely randomized design pattern (CRD) with a single factor divided into 5 levels with 6 replications. Variables observed include the time of buds, number of shoots, plant height, time of leaves, number of leaves, time of roots, number of roots, root length. The results showed the composition of 25% MS + 75% Growmore gave the highest results on the number of shoots, the composition of 50% MS + 50% gave the best results on plantlet height, number of leaves, and number of roots.